Journal: Yonsei Medical Journal

Article Title: LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p/EZH2 Axis

doi: 10.3349/ymj.2021.62.7.640

Figure Lengend Snippet: LINC00662 expression was up-regulated in OSCC tissues and cells. (A) qRT-PCR was employed for detecting the expression of LINC00662 in OSCC tissues and adjacent normal tissues. (B) qRT-PCR was adopted for assaying the expression of LINC00662 in NOK cells, OSCC cells (SCC-25, ISG15, SCC-9, and CAL-27), and gastric cancer cell line AGS. (C) The expression level of LINC00662 in OSCC tissues with early stage (I–II) or advanced stage (III–IV) was measured. (D) The expression level of LINC00662 was detected in OSCC tissues with or without lymph node metastasis. ** p <0.01, *** p <0.001, and NS: p >0.05. OSCC, oral squamous cell carcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; NOK, normal oral keratinocyte; AGS, human gastric adenocarcinoma cell.

Article Snippet: OSCC cell lines (SCC-25, ISG15, SCC-9, and CAL-27), normal oral keratinocyte (NOK) cell line and human gastric adenocarcinoma cell (AGS) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Yonsei Medical Journal

Article Title: LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p/EZH2 Axis

doi: 10.3349/ymj.2021.62.7.640

Figure Lengend Snippet: LINC00662 targeted miR-144-3p in OSCC cells. (A) Bioinformatics analysis was employed for predicting the binding sequence between miR-144-3p and LINC00662. Red-colored letters indicate complementary base pairing. (B and C) The luciferase activity in SCC-9 and ISG15 cells co-transfected with LINC00662-WT or LINC00662-MUT, and miR-control or miR-144-3p mimics was assayed using dual-luciferase reporter gene assay. (D and E) RIP assay was used to validate the direct binding relationship between LINC00662 and miR-144-3p in OSCC cells. (F) qRT-PCR was utilized for quantifying the expression of miR-144-3p in OSCC tissues and adjacent normal tissues. (G) qRT-PCR was used for assaying the expression of miR-144-3p in NOK cells, OSCC cells (SCC-25, ISG15, SCC-9, and CAL-27), and AGS cells. (H) Pearson correlation analysis was employed for evaluating the correlation between miR-144-3p and LINC00662 expression levels in OSCC tissue. (I and J). The expression of LINC00662 or miR-144-3p in SCC-9 and ISG15 cells with LINC00662 overexpression was detected employing qRT-PCR. ** p <0.01, *** p <0.001, and NS: p >0.05. WT, wild type; MUT, mutant type; IgG, immunoglobulin G; NOK, normal oral keratinocyte; OSCC, oral squamous cell carcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; RIP, RNA immunoprecipitation; AGS, human gastric adenocarcinoma cell.

Article Snippet: OSCC cell lines (SCC-25, ISG15, SCC-9, and CAL-27), normal oral keratinocyte (NOK) cell line and human gastric adenocarcinoma cell (AGS) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Binding Assay, Sequencing, Luciferase, Activity Assay, Transfection, Control, Reporter Gene Assay, Quantitative RT-PCR, Expressing, Over Expression, Mutagenesis, Real-time Polymerase Chain Reaction, RNA Immunoprecipitation

Journal: Yonsei Medical Journal

Article Title: LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p/EZH2 Axis

doi: 10.3349/ymj.2021.62.7.640

Figure Lengend Snippet: The up-regulation of LINC00662 expression facilitated proliferation, migration, invasion, and EMT of OSCC cells by inhibiting miR-144-3p expression. (A) qRT-PCR was used to detect the expression of miR-144-3p in OSCC cells transfected with LINC00662 overexpression plasmid or co-transfected with LINC00662 overexpression plasmid and miR-144-3p mimics, respectively. (B) The proliferation of transfected cells was detected by CCK-8 method. (C and D) Transwell experiment was used to detect the migration and invasion of transfected cells. (E) Protein expression levels of E-cadherin and Vimentin in transfected cells were detected by Western blot. * p <0.05, ** p <0.01, *** p <0.001. EMT, epithelial-mesenchymal transition; qRT-PCR, quantitative real-time polymerase chain reaction; OSCC, oral squamous cell carcinoma; CCK-8, Cell counting kit-8.

Article Snippet: OSCC cell lines (SCC-25, ISG15, SCC-9, and CAL-27), normal oral keratinocyte (NOK) cell line and human gastric adenocarcinoma cell (AGS) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Expressing, Migration, Quantitative RT-PCR, Transfection, Over Expression, Plasmid Preparation, CCK-8 Assay, Western Blot, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Yonsei Medical Journal

Article Title: LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p/EZH2 Axis

doi: 10.3349/ymj.2021.62.7.640

Figure Lengend Snippet: The regulation of LINC00662/miR-144-3p on EZH2 expression. (A-D) qRT-PCR and Western blot were used to detect the expression level of EZH2 mRNA and protein in SCC-9 and ISG15 cells after the overexpression of miR-144-3p or LINC00662. (E) qRT-PCR was used to detect the expression of EZH2 mRNA in OSCC tissues and adjacent normal tissues. (F and G) Pearson correlation analysis was used to detect the correlation between EZH2 mRNA expression level and miR-144-3p expression level or LINC00662 expression level in OSCC tissues. *** p <0.001. EZH2, enhancer of zeste homolog 2; qRT-PCR, quantitative real-time polymerase chain reaction; OSCC, oral squamous cell carcinoma.

Article Snippet: OSCC cell lines (SCC-25, ISG15, SCC-9, and CAL-27), normal oral keratinocyte (NOK) cell line and human gastric adenocarcinoma cell (AGS) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression, Real-time Polymerase Chain Reaction

Journal: Yonsei Medical Journal

Article Title: LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p/EZH2 Axis

doi: 10.3349/ymj.2021.62.7.640

Figure Lengend Snippet: Silencing EZH2 could reverse the cancer-promoting effects of LINC00662 in OSCC. (A and B) Western blot was used to detect the expression of EZH2 protein in SCC-9 and ISG15 cells transfected with LINC00662 overexpression plasmid or co-transfected with LINC00662 overexpression plasmid and si-EZH2, respectively. (C) Western blot was used to detect the protein expression levels of E-cadherin and Vimentin in transfected cells. (D and F) The proliferation, migration, and invasion of transfected cells were detected by CCK-8 method and Transwell experiment, respectively. * p <0.05, ** p <0.01, *** p <0.001. EZH2, enhancer of zeste homolog 2; OSCC, oral squamous cell carcinoma; CCK-8, Cell counting kit-8.

Article Snippet: OSCC cell lines (SCC-25, ISG15, SCC-9, and CAL-27), normal oral keratinocyte (NOK) cell line and human gastric adenocarcinoma cell (AGS) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Western Blot, Expressing, Transfection, Over Expression, Plasmid Preparation, Migration, CCK-8 Assay, Cell Counting

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: SHMT2 is up-regulated in OSCC cells. (a-b) The detection of SHMT2 mRNA and protein levels employed RT-qPCR and western blot in OSCC cell lines. *P < 0.05, ***P < 0.001 vs. HOK.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Quantitative RT-PCR, Western Blot

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 binds to SHMT2 and can be downregulated by SHMT2 silencing in OSCC cells. (a-b) The interaction between SHMT2 and ILF2 was found through the MINT and BioGRID databases. (c) ILF2 level in oral tumor tissue was analyzed using TNMplot database. (d-e) RT-qPCR and western blot analysis of ILF2 expression. **P < 0.01, ***P < 0.001 vs. HOK. (f) ILF2 expression in SHMT2-knockdown CAL-27 cells was detected with the use of western blot. ***P < 0.001 vs. si-NC. (g) The interaction of SHMT2 and ILF2 was identified using Co-IP.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Knockdown, Co-Immunoprecipitation Assay

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 upregulation reverses the impacts of SHMT2 knockdown on the occurrence of OSCC. (a-b) RT-qPCR and western blot analysis of the overexpression efficiency of ILF2. ***P < 0.001 vs. Oe-NC. (c) CCK-8 assay appraised cell viability in CAL-27 cells transfected with si-SHMT2 and Oe-ILF2. (d) Cell proliferation was identified utilizing colony formation assay in CAL-27 cells transfected with si-SHMT2 and Oe-ILF2. (e-f) TUNEL assay appraised cell apoptosis. (g-h) Western blot analysis of the expression of apoptosis-related factors. **P < 0.01, ***P < 0.001 vs. si-NC; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. si-SHMT2+ Oe-NC.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Over Expression, CCK-8 Assay, Transfection, Colony Assay, TUNEL Assay, Expressing

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 elevation restores the impacts of SHMT2 knockdown on OSCC cell migration, invasion and EMT. (a-b) Wound healing and transwell assays appraised cell migration and invasion. (c) Western blot analysis of MMP2 and MMP9 expression. (d) Western blot analysis of the expression of EMT-related factors. ***P < 0.001 vs. si-NC; ## P < 0.01, ### P < 0.001 vs. si-SHMT2+ Oe-NC.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Knockdown, Migration, Western Blot, Expressing

Journal: Aging (Albany NY)

Article Title: Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma

doi: 10.18632/aging.204089

Figure Lengend Snippet: Clinical relevance of histamine receptor H1 (HRH1) levels in head and neck squamous cell carcinoma (HNSCC) or oral squamous cell carcinoma (OSCC) patients obtained from TCGA and GEO databases. ( A , B ) HRH1 expression was higher in HNSCC tissues ( A ) and OSCC tissues ( B ) than in normal tissues (database sources: TCGA and GSE78060). ( C ) HRH1 expression levels in OSCC from GSE78060 were compared according to the tumor size (T stages). ( D ) Kaplan-Meier curves for overall and disease-specific survival (left panel) and recurrence-free survival (right panel) of patients with HNSCC and OSCC, as categorized according to high or low expression of HRH1 . The p value indicates a comparison between patients with HRH1 high and HRH1 low . (database sources: TCGA and GSE31056).

Article Snippet: The human SAS, HSC-3, HSC-3M, and SCC9 OSCC cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan) or the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma

doi: 10.18632/aging.204089

Figure Lengend Snippet: Correlations of histamine receptor H1 (HRH1) rs346074 and rs901865 genotypes with HRH1 protein levels in five oral squamous cell carcinoma (OSCC) cell lines. Upper panel, HRH1 rs346074 or rs901865 genotypes in OSCC cells (SAS, HSC-3M, HSC-3, SCC9, and OECM1) were detected by a TaqMan SNP Genotyping Assay. Lower panel, protein levels of HRH1 were detected by a Western blot analysis using two HRH1-specific antibodies.

Article Snippet: The human SAS, HSC-3, HSC-3M, and SCC9 OSCC cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan) or the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: TaqMan SNP Genotyping Assay, Western Blot